Laboratory Manual for Genetic Engineering

This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering. The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens. This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology. Table of Contents Preface. Isolation of Genomic DNA from Gram Negative, Gram Positive Bacteria, Blood and Mammalian Tissue. Isolation of Plasmid DNA from Gram Negative and Gram Positive Bacteria. Isolation of RNA from Bacteria and Cultured Mammalian Cells. Estimation of Nucleic Acids. Restriction Digestion and Ligation of DNA. Polymerase Chain Reaction and Randomly Amplified Polymorphic DNA. Electrophoresis